In ELISA applications, the substrate PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is frequently employed to detect alkaline phosphatase. PNPP and alkaline phosphatase react to produce a yellow, water-soluble reaction product. At 405 nm, this reaction product absorbs light. Pierce PNPP comes in four different forms: dry crystalline powder, single-use tablets, a stable substrate solution, and a whole kit that includes both the substrate solution and the diethanolamine buffer.
PNPP Substrate Tablets
In ELISA applications, the substrate PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is frequently employed to detect alkaline phosphatase. PNPP and alkaline phosphatase react to produce a yellow, water-soluble reaction product. At 405 nm, this reaction product absorbs light. Pierce PNPP comes in four different forms: dry crystalline powder, single-use tablets, a stable substrate solution, and a whole kit that includes both the substrate solution and the diethanolamine buffer.
