Learn more about PNPP Substrates, the dependable and premium enzyme substrates made for use in a range of biochemical tests and research applications. P-nitrophenyl phosphate (PNPP) substrates are frequently employed in enzymatic processes to identify and measure the activity of particular enzymes. In tests for alkaline phosphatases and other enzymes, these substrates ensure reliable and repeatable findings because to their exceptional sensitivity and stability. The test procedure is made simpler and important research time is saved by PNPP Substrates' user-friendly methods and optimised formulations. PNPP Substrates produce accurate and repeatable findings in many types of research, including that conducted in academia, pharmaceutical development, and clinical diagnostics.
In ELISA applications, the substrate PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is frequently employed to detect alkaline phosphatase. PNPP and alkaline phosphatase react to produce a yellow, water-soluble reaction product.
At 405 nm, this reaction product absorbs light. Pierce PNPP comes in four different forms: dry crystalline powder, single-use tablets, a stable substrate solution, and a whole kit that includes both the substrate solution and the diethanolamine buffer.
• Maximum absorbance of 405 nm; one-component alkaline phosphatase (AP) substrate.
When used with AP, it: reacts to produce a yellow colour in solution; • Is safer to use because it has a non-mercury, non-azide preservative.
Form in powder.
5 mg pills with the remainder being an inert filler.
By offering a single component PNPP substrate, avoids this laborious and unpredictable introduction phase.
This substrate can be stopped using standard techniques and is stable for a year between 2 and 8 degrees Celsius.