GRAM'S SAFRANINE SOLUTION FOR THE GRAM STAINI

Code: 1.09217.0500

That enables differentiating the blue and purple-colored Gram-positive bacteria from the red-colored Gram-negative bacteria.

per 500ML
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Synonyms: Safranin T , Safranin Y , 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride , Safranin O , Gossypimine , CI 50240 , Basic Red 2 , Cotton Red , Safranin A , Safranin M-Clarity™ quality level = MQ300 Counterstain for Gram staining The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set. It is an IVD product and CE registered.

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